Duration: 60 minutes
Max. Number of Participants: 15
Summary:
In this workshop, students will have the opportunity to perform DNA cleavage using a restriction endonuclease—an enzyme that cuts DNA molecules into smaller fragments by recognizing specific short nucleotide sequences. The resulting fragments will be separated using gel electrophoresis, and the generated images will be photographed and analyzed.
Participants will learn under what conditions restriction endonucleases cut DNA, their origins, and how they have contributed to the development of modern molecular cloning technologies and forensic genetics. The program includes hands-on experience with micropipettes, enzymes, and electrophoresis—a technique used to separate DNA fragments based on size.